Innovation in Making Buffalo (Bubalus bubalis) Milk Probiotic: Dadih
DOI:
https://doi.org/10.6000/1927-520X.2026.15.01Keywords:
Bamboo, buffalo milk, dadih, fermentation, probioticAbstract
Dadih is a traditional, sustainable food made from fermented buffalo milk, passed down from generation to generation on the island of Sumatera for thousands of years. The COVID-19 outbreak has significantly increased demand for buffalo milk, including fermented buffalo milk/dadih in Sumatera due to its nutritional content. Until now, milk fermentation has been carried out using Bambusa verticillata bamboo tube containers. This fermentation process has varied consequences depending on the conditions of each bamboo. Furthermore, bamboo cannot be reused in making dadih, so efforts are underway to find alternative containers for producing dadih, including plastic containers with the addition of a starter culture. This study aimed to identify the optimal conditions for manufacturing dadih in plastic containers using bamboo dadih as the initial culture. The study utilised a factorial completely randomized design comprising two factors. The first factor was the fermentation period, which was 24 to 48 hours. The second factor comprised initial concentrations of 1.5%, 3%, 4.5%, and 6%.
At the beginning of the study, dadih was isolated to obtain a dominant culture, which was then purified and identified using the PCR 16S rRNA method-the study analysed chemical quality parameters, including pH, protein content, and total acid. In addition, it evaluated the biological condition by quantifying the overall number of bacterial colonies. The organoleptic characteristics were assessed based on aroma, color, texture, and taste. The PCR results showed that the dominant bacteria were Weissella cibaria. The pH levels across all treatments ranged from 4.53 to 4.62.
Furthermore, the protein level decreased as the proportion of starter and the length of fermentation increased. In contrast, the overall acidity level rose as the proportion of starter and duration of fermentation increased. There was an interaction between the fermentation period and dadih concentration. The organoleptic test showed significant differences in texture and taste due to the treatment using a 3% starter and a fermentation time of 24 hours. The study results suggest that fermenting dadih in plastic containers with a 3% starter for 24 hours produces the most desirable organoleptic effects. The colony count of 2.4x108, which is dominantly by Weissela cibaria, indicates that the drink is classified as probiotic.
References
Borghese A, Chiariotti A, Barile VL. Buffalo in the world: situation and perspectives. In: Borghese A, editor. Biotechnological applications in buffalo research. Singapore: Springer 2022. p. 3-31.
Becskei Z, Savić M, Ćirković D, Rašeta M, Puvača N, Pajić M, et al. Assessment of water buffalo milk and traditional milk products in a sustainable production system. Sustainability 2020; 12(16): 6616.
Lakhanpal J, Gupta J. Decreased antibiotic susceptibility and enhanced probiotic production potential of buffalo milk fermented dadih compared to cow and buffalo milk. BioTechnologia 2017; 98: 1-9.
Rai DC, Rathaur A, Yadav AK. Nutritional and nutraceutical properties of buffalo milk for human health: a review. Indian J Dairy Sci 2022; 75: 1-10.
Pato U. The potential of lactic acid bacteria isolated from dadih to reduce cancer risk. J NaturIndones 2003; 5: 162-6.
Veettil VN. Optimization of bacteriocin production by Lactobacillus plantarum using response surface methodology. Cell Mol Biol 2022; 68: 105-10.
Effendi E, Festiyed F, Azhar M, Andromeda A, Yerimadesi Y. Dadiah: a highly nutritious probiotic drink typical of Minangkabau and ethno science-based science learning resources. In: AIP Conf Proc 2023; 2673: 012001.
Food and Agriculture Organization of the United Nations, World Health Organization. Guidelines for the evaluation of probiotics in food. Working Group Report. Washington (DC): FAO/WHO 2002.
Mustopa AZ, Fatimah F. Diversity of lactic acid bacteria isolated from Indonesian traditional fermented foods. Microbiology Indonesia 2014; 8(2): 2-2.
Collado MC, Surono I, Meriluoto J, Salminen S. Potential probiotic characteristics of Lactobacillus and Enterococcus strains isolated from traditional dadih fermented milk against intestinal colonisation pathogens. J Food Prot 2007; 70: 700-5.
Ginting N, Yunilas Y, Mirwandhono RE, Lin YY. Probiotic ice cream using buffalo milk dadih: microbial, chemical, and sensory characteristics. J Adv Vet Anim Res 2025; 12(1): 214.
Chromý V, Vinklárková B, Šprongl L, Bittová M. The Kjeldahl method as a primary reference procedure for total protein in certified reference materials used in clinical chemistry: a review. Crit Rev Anal Chem 2015; 45(2): 106-11.
Feldsine P, Abeyta C, Andrews WH. AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. J AOAC Int 2002; 85(5): 1187-200.
Popper R, Kroll JJ. Consumer testing of food products using children. In: Developing children’s food products. Cambridge: Woodhead Publishing 2011; 163-87.
Pourahmad R, Mazaheri Assadi M. Yoghurt production by Iranian native starter cultures. Nutr Food Sci 2005; 35: 410-5.
Setiarto RHB, Anshory L, Wardana AA. Nutritional and microbiological characteristics of dadih and their application to the food industry: a review. In: IOP Conf Ser Earth Environ Sci 2023; 1252(1): 012153.
Fatdillah H, Febrianti F, Sari DR. The effect of spontaneous fermentation and back-slopping on the quality of curd based on total lactic acid bacteria, pH, dan total titratable acidity. J Riset RumpunIlmu Hewani 2023; 2(2): 69-81.
Ahmad S. Buffalo milk. In: Milk and dairy products in human nutrition: production, composition and health. Oxford: Wiley-Blackwell 2013: 519-53.
Jatmiko YD, Howarth GS, Barton MD. Evaluation of yeast diversity in dadih and dangke using PCR-RFLP of the internal transcribed spacer region. In: IOP Conf Ser Earth Environ Sci 2019; 391(1): 012025.
Horstman AM, Huppertz T. Milk proteins: Processing, gastric coagulation, amino acid availability and muscle protein synthesis. Critical Reviews in Food Science and Nutrition 2023; 63(30): 10267-10282.
Arnold M, Rajagukguk YV, Gramza-Michałowska A. Characterization of dadih: traditional fermented buffalo milk of Minangkabau. Beverages 2021; 7(3): 60.
Helmizar H, Yuswita E, Putra A. Analysis of the nutrients and microbiological characteristics. Glob J Health Sci 2019; 11(1): 1-10.
Usmiati S, Risfaheri R. Dadih has been developed as a functional probiotic food native to West Sumatra. J Agric Res Dev 2013; 32: 309-15.
Hawaz E. Isolation and identification of probiotic lactic acid bacteria from dadih and in vitro evaluation of its growth inhibition activities against pathogenic bacteria. Afr J Microbiol Res 2014; 8: 1419-25.
Moreno-Montoro M, Navarro-Alarcón M, Bergillos-Meca T, Giménez-Martínez R, Sánchez-Hernández S, Olalla-Herrera M. Physicochemical, nutritional, and organoleptic characterisation of skimmed buffalo milk fermented with the probiotic strain Lactobacillus plantarum C4. Nutrients 2018; 10: 633.
Hallqvist J. The importance of buffalo milk in the dadih manufacture of Sri Lanka [master’s thesis]. Uppsala: Swedish University of Agricultural Sciences 2019.
Herlina VT, Setiarto RHB. From tradition to innovation: dadih, the Minangkabau tribe’s traditional fermented buffalo milk from Indonesia. J Ethn Foods 2024; 11(1): 21.
Ginting N. Dadih bamboo ampel (Bambusa vulgaris) and bamboo gombong (Gigantochloaverticillata) fermented for 2 and 3 days: effect on salad dressing hedonic quality. In: IOP Conf Ser Earth Environ Sci 2018; 130(1): 012029.
Fitri A, Asra R, Rivai H. Overview of the traditional, phytochemical, and pharmacological uses of gold bamboo (Bambusa vulgaris). World J Pharm Pharm Sci 2020; 9: 299-318.
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